![]() Of 313 T-SPOT.TB assays, 235 were performed with fresh cells and 78 were performed with frozen cells. ![]() ![]() Culture plates were read visually by four different observers from two laboratories and by two automated readers. The study was nested within an ongoing cohort study, in which part of the T-SPOT.TB had been performed with frozen material. Our aim was to evaluate variation between different observers in reading T-SPOT.TB results. T-SPOT.TB has been used in various clinical-epidemiological settings, but so far no studies have evaluated the effect of interobserver variation in test reading. The assay needs to be performed with freshly isolated cells, and interpretation requires training. ![]() ABSTRACT T-SPOT.TB is a specific assay for the diagnosis of tuberculosis.
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